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KMID : 0855520070200010021
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Korean Journal of Physical Anthropology
2007 Volume.20 No. 1 p.21 ~ p.34
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The Morphometric Changes of the Enteric Nervous System in the Intestinal Transection-reanastomosis Model
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Oh Soo-Yoon
Park Ung-Chae
Kim Kyung-Rae
Wang Joon-Ho
Lee Jae-Dong
Kim Soon-Je
Song Wu-Chul
Park Seung-Hwa
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Abstract
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This study was performed to investigate the morphometric changes of myenteric plexus and type 1 interstitial cells of Cajal (ICC-I) in regeneration process of small intestine transection.
Sprague Dawley rats (200~250 g) were anesthetized with ether; then the full thickness of ileal wall were semitransected; and subsequent end-to-end anastomosis were performed by using 6-0 silk suture thread. Sham-operated rats, which only underwent the laparotomy, were used for control group. Experimental animals were sacrificed at 3 days, 7 days, 15 days, and 30 days after the operation. In each group myenteric plexus and ICC-Is were prepared by histochemical method (NADH-TR stain for myenteric plexus, ZIO stain for ICC-Is) and cell numbers were counted by image analyzer (Image plus pro-5.0, Media Cybermedics, USA).
Degeneration of myenteric neurons and ICC-Is occurred simultaneously and it was similar in oral and anal to the site of transection. Degeneration effects were most prominent at 3 days and 7 days after intestinal transection. In myenteric plexus, many neurons had degenerated appearances and about 40% of them were lost. Most of ICC-Is had cytoplasmic vacuoles and 20~37% of the cells were lost. At 15 days after transection, there were no more degeneration in myenteric neurons (20~25% cell loss) and ICC-Is (20~13% cell loss). At 30 days after transection, numbers of myenteric neurons were not recovered as that of the control group. However numbers of ICC-Is were as similar as that of the control group.
In conclusion, we confirmed that degeneration effects of intestinal transection are more severe in myenteric plexus than in ICC-Is, and recovery of cell loss occurs more slowly in myenteric plexus.
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KEYWORD
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Intestinal transection-reanastomosis, Myenteric plexus, Type 1 interstitial cells of Cajal(ICC-Is), Regeneration, Morphometry
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